pstat1 y701 (9167) Search Results


pstat1 y701 (9167)  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc pstat1 y701 (9167)
STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of <t>phospho-STAT1</t> protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.
Pstat1 Y701 (9167), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pstat1 y701 (9167)/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
pstat1 y701 (9167) - by Bioz Stars, 2026-02
90/100 stars
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rabbit anti pstat1 y701  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc rabbit anti pstat1 y701
STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of <t>phospho-STAT1</t> protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.
Rabbit Anti Pstat1 Y701, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti pstat1 y701/product/Cell Signaling Technology Inc
Average 98 stars, based on 1 article reviews
rabbit anti pstat1 y701 - by Bioz Stars, 2026-02
98/100 stars
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pstat1  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc pstat1
STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of <t>phospho-STAT1</t> protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.
Pstat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pstat1/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
pstat1 - by Bioz Stars, 2026-02
99/100 stars
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il-6 12153 antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc il-6 12153 antibody
STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of <t>phospho-STAT1</t> protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.
Il 6 12153 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il-6 12153 antibody/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
il-6 12153 antibody - by Bioz Stars, 2026-02
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pstat1 y701 24 h cell signaling ab  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc pstat1 y701 24 h cell signaling ab
STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of <t>phospho-STAT1</t> protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.
Pstat1 Y701 24 H Cell Signaling Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pstat1 y701 24 h cell signaling ab/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
pstat1 y701 24 h cell signaling ab - by Bioz Stars, 2026-02
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elf4a1 (2490)  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc elf4a1 (2490)
STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of <t>phospho-STAT1</t> protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.
Elf4a1 (2490), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elf4a1 (2490)/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
elf4a1 (2490) - by Bioz Stars, 2026-02
90/100 stars
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pstat1 y701  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc pstat1 y701
STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of <t>phospho-STAT1</t> protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.
Pstat1 Y701, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pstat1 y701/product/Cell Signaling Technology Inc
Average 98 stars, based on 1 article reviews
pstat1 y701 - by Bioz Stars, 2026-02
98/100 stars
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total stat1  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc total stat1
STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of <t>phospho-STAT1</t> protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.
Total Stat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total stat1/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
total stat1 - by Bioz Stars, 2026-02
94/100 stars
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actin antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc actin antibody
STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of <t>phospho-STAT1</t> protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.
Actin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/actin antibody/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
actin antibody - by Bioz Stars, 2026-02
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mmp-12  (Triple Point Biologics)
90
Triple Point Biologics mmp-12
STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of <t>phospho-STAT1</t> protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.
Mmp 12, supplied by Triple Point Biologics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mmp-12/product/Triple Point Biologics
Average 90 stars, based on 1 article reviews
mmp-12 - by Bioz Stars, 2026-02
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cd36 abcam ab ab137320  (Danaher Inc)
86
Danaher Inc cd36 abcam ab ab137320
STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of <t>phospho-STAT1</t> protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.
Cd36 Abcam Ab Ab137320, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pstat1 s727  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc pstat1 s727
STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of <t>phospho-STAT1</t> protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.
Pstat1 S727, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pstat1 s727/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
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Image Search Results


STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of phospho-STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.

Journal: Cancer Biology & Therapy

Article Title: Antibody dependent cell-mediated cytotoxicity selection pressure induces diverse mechanisms of resistance

doi: 10.1080/15384047.2023.2269637

Figure Lengend Snippet: STAT1 signaling is associated with but does not drive acquisition of ADCC resistance (a), western blot analysis of STAT1 pathway proteins in ADCC-sensitive (ADCCS) and ADCC-resistant (ADCCR) A431, SKOV3, and FaDu cells. Densitometry values for expression normalized to GAPDH in ADCC-resistant relative to ADCC- sensitive cells are indicated. (b), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or medium supplemented with DMSO (horizontal hash bars) or 10 nM ruxolitinib (diagonal hash bars) for 72hrs prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, ns, not significant. Error bars, SEM. (c), Representative western blot analysis of phospho-STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or medium supplemented with DMSO or 10 nM ruxolitinib for 72hrs. Densitometry values for expression normalized to GAPDH in cells treated with DMSO or ruxolitinib compared to untreated cells are indicated. (d), percent cytotoxicity of ADCC-sensitive (blue) and ADCC-resistant (red) A431 cells as measured by ADCC assay when cells are incubated in either medium alone (solid bars) or transfected with control scramble siRNA (siNEG, horizontal hash bars) or siRNA specific for STAT1 (diagonal hash bars) for 48 h prior to ADCC assay ( n = 3). Unpaired two-tailed t -test, **, P <.01. Error bars, SEM. (e), Representative western blot analysis of STAT1 protein expression in ADCC-sensitive and ADCC-resistant A431 cells when cells are incubated in either medium alone (untreated) or transfected with control scramble siRNA (siNEG) or siRNA specific for STAT1 for 48hrs. Densitometry values for expression normalized to GAPDH in cells transfected with control scramble (siNEG) or siRNA specific for STAT1 compared to untreated cells are indicated. (f), western blot analysis of STAT1 protein expression in untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at the indicated challenges. (g), percent cytotoxicity of untreated and ADCC condition treated parental A431 cells, A431 cells transduced with a non-targeting control CRISPR-cas9 (NTC), and A431 cells transduced with CRISPR-cas9 specific for STAT1 (STAT1-KO) at every 5 challenges during derivation of resistance as measured by ADCC assay ( n = 2). Unpaired two-tailed t- test, *, P <.05. Error bars, SEM.

Article Snippet: Western blots were conducted with Cell Signaling antibodies to: E-Cadherin (3195), Vimentin (5741), Snail (3879), Twist (46702), EGFR (4267), pEGFR Y1068 (3777), pEGFR Y1045 (2237), HER2 (4290), pHER2 Y1248 (2247), ERK1/2 (4695), pERK1/2 T202/Y204 (4370), AKT (pan) (4691), pAKT S473 (4060), CD54 (65055), STAT1 (14994), pSTAT1 Y701 (9167), M×1(37849), IL-6 (12153), ATR (2790), pCHK1 S345 (2341), PSMB8 (13635), and PSMB10 (78385) at concentrations of 1:1000 diluted in 5% milk in TBST.

Techniques: Western Blot, Expressing, ADCC Assay, Incubation, Two Tailed Test, Transfection, Transduction, CRISPR